| ORIGINAL ARTICLE |
|
| Year : 2019 | Volume
: 6
| Issue : 2 | Page : 49-55 |
|
|
Analysis of spontaneous depolarization-linked hyperpolarizations in mouse detrusor smooth muscle cells
Mithun Padmakumar1, Keith L Brain2, Rohit Manchanda1
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India
2 Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
Correspondence Address:
Mithun Padmakumar
Room No. 506, Fifth Floor, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai - 400 076, Maharashtra
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/BMRJ.BMRJ_9_19
|
|
|
Background: Urinary bladder detrusor smooth muscle cells exhibit spontaneous electrical activities comprising various signal types. Aims and Objectives: This article introduces and analyzes a rare category of signals observed in such activity, named spontaneous depolarization-linked hyperpolarization (sDLH). Materials and Methods: A mouse model was used in the study, where all the occurrences of sDLHs were pooled together from multiple intracellular recording sessions. Four features – (i) resting membrane potential (RMP) (R, in mV), (ii) depolarization amplitude (D, in mV), (iii) hyperpolarization amplitude (H, in mV), and (iv) time course of the hyperpolarization (T, in ms) – were evaluated from all sDLHs. Results: The analysis of results indicated that (a) the signals appear more frequently in cells with higher RMP, (b) the depolarization amplitudes seem to be distributed randomly and have no correlation with other features, (c) hyperpolarization amplitudes show two distinct clusters and exhibit strong correlation with the RMP, and (d) time course of hyperpolarization phase shows no distinct groups and is distributed in a window larger than that of any other signals seen in the intracellular recordings. With the help of the results obtained from the analysis, a hypothesis for the biophysical origin of these signals is proposed. Conclusions: This needs to be tested experimentally, and if proved right, would help extend the boundaries of our current understanding of the detrusor smooth muscle system.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
